Investigación
Research

Metabolomics Laboratory (CEMBIO)

Metabolomics Laboratory (CEMBIO)

Laboratorio De Metabolómica (Cembio)
Red de laboratorios de la Universidad San Pablo CEU Red de laboratorios de la Comunidad de Madrid

Nº de registro: 260

Responsable

Dra. Antonia García Fernández

Ubicación

Laboratory 1.06. Building B

School of Pharmacy

Universidad CEU San Pablo

Urb. Montepríncipe. 28925

Alcorcón, Madrid 

Personal

Senior Officer specialised in mass spectrometry: Ms. Vanesa Alonso Herranz.

Web:

Contacto:

Teléfono: +34 913724753/69

Servicios

Quantitative analyses (targeted analysis) of one or several selected metabolites on all sorts of biological samples: blood, serum, plasma, urine, cerebrospinal fluid, saliva, tissues, cells, culture means by high-resolution separation methods such as liquid chromatography, gas chromatography, or capillary electrophoresis, coupled to mass spectrometry as a detection system. It includes the whole samples treatment process, from the reception up to the final report, according to the client’s needs. Advisory and recommendations on the most adequate technique depending on the metabolite’s assessment and sample.

Non-targeted Analysis of Metabolites in all the biological sample. It is about performing a differential analysis of biological samples (blood, serum, plasma, urine, cerebrospinal fluid, saliva, tissues, or cells culture) by applying high-resolution separation methods such as liquid chromatography, gas chromatography, or capillary electrophoresis coupled to mass spectrometry detection systems, with the aim of obtaining metabolomic profiles. These profiles, subject to an appropriate comparative analysis between problem and control samples, can cause the identification of biomarkers of interest. Their multiples applications: pathophysiological processes related to several diseases, drugs development, toxicity analysis, diagnosis and prognosis markers development, or even the environmental changes evaluation. The comprehensive study they offer includes: sample treatment, analysis of different methods, data reprocessing, statistical analysis, and biochemical interpretation, being able to coordinate analytical and biochemical aspects, or purely analytical ones.

Optimisation and assessment of analytical methods.

Analysis of preservatives, active ingredients or other compounds of interest due to HPLC-UV in cosmetic and/or pharmaceutical products.

Equipamientos

ANALYSIS OF BIOLOGICAL SAMPLES DUE TO HIGH-RESOLUTION METHODS:

  1. LIQUID CHROMATOGRAPHY (LC) ANALYSIS WITH MASS SPECTROMETRY (MS) DETECTION WITH DIFFERENT ANALYSERS (AGILENT TECHNOLOGIES)
    • Liquid chromatography equipment coupled to a quadrupole-time-of-flight mass spectrometry detector. LC-MS-QTOF: 1290 Infinity II LC coupled to a 6545 Q-TOF detector. Equipment offering EXACT MASS. Tandem mass spectrometry.
    • Liquid chromatography equipment coupled to a quadrupole-simple-time mass spectrometry detector. LC-MS-QqQ. LC 1260 Infinity system coupled to a detector 6470 Triple Quad. Equipment offering the maximum sensitivity and selectivity. Tandem mass spectrometry.
  1. GAS CHROMATOGRAPHY (GC) ANALYSIS WITH MASS SPECTROMETRY (MS) DETECTION WITH DIFFERENT ANALYSERS (AGILENT TECHNOLOGIES)
  • Gas chromatography equipment coupled to a quadrupole-time-of-flight mass spectrometry detector. GC-MS-QTOF. 7890B GC System coupled to a detector 7250 Q-TOF. Equipment offering EXACT MASS. Tandem mass spectrometry.
  • Gas chromatography equipment coupled to a quadrupole-simple-time mass spectrometry detector. 8890 GC System coupled to a detector 5977B Q.
  1. CAPILLARY ELECTROPHORESIS ANALYSIS WITH MASS SPECTROMETRY DETECTION AND EXACT MASS ANALYSER (AGILENT TECHNOLOGIES)
    • Two capillary electrophoresis equipments coupled to a quadrupole-time-of-flight mass spectrometry detectors. 7100 Capillary electrophoresis coupled to a detector 6224 TOF MS and a detector 6230 TOF MS. Equipments offering EXACT MASS.

MASS PREPARETION EQUIPMENT: 

  • Lyophilizer (CoolSafe Pro110-4 Freeze Dryer)
  • Centrifugal evaporator (HyperVAC model VC2124 centrifuge vacuum concentrator and Savant SPD121P Speed Vac)
  • Homogenisers: Tissuelyser LT, cytogenic mortar (Covaris cryoPREP)
  • Centrifuges and ultra-microcentrifuges

Tarifas