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Specialised parasitological diagnosis in human, animal and environmental samples:
Microsporidia: Modified trichrome stain, IFA and PCR.
Cryptosporidium: PCR and specific staining.
Cyclospora: Safranin stain and PCR.
Entamoeba histolytica: PCR.
Diagnosis of visceral and ocular Toxocariosis by ELISA and WB.
Malaria: immunochromatography.
Free-living amoebae: Acanthamoeba: Tissue staining, IFA, Real Time-PCR and culture.
Balamuthia: Tissue staining, IFA, Real Time-PCR and culture.
Naegleria: Real Time-PCR.
Parasite detection in environmental samples (water) following EPA 1623 regulations for Cryptosporidium and Giardia. With this methodology we also offer the detection of free-living amoebae, microsporidia and Cyclospora.
Equipamientos
Nikon brightfield and immunofluorescence microscopes with image analysis system (Analysis®), microphotography and Normarsky phase contrast.
Parasite culture: Type II biological safety hoods (Faster BHA-36®), multi-temperature culture ovens with and without CO2 (Controltecnica, Heracell®).
Biosafety centrifuges with autoclavable baskets (Megafuge 2.0R®, Heraeus).
Microcentrifuges (Biofuge primo R®, Heraeus) and shaker-disruptor (Fast-Prep 24®, MP Biomedicals).
Water baths, heating plates, orbital and rocker shakers. PHmeters, balances.
Refrigerators and freezers of -40ºC and -80ºC.
Complete equipment for PAGE and WB transfer.
ELISA plate readers.
PCR System 9700 Gene Amp® Thermocyclers, Real Time PCR: Stratagene Mx3000P® (Agilent Technologies).
Water concentration system: IDEEX Filta-Max® Automatic - FMC 11003.
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